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The Thermo Fisher 7500 Fast Real-Time PCR System is a versatile and high-speed quantitative PCR platform designed for fast cycling protocols, delivering results in under 40 minutes. Ideal for gene expression, genotyping, pathogen detection, and viral load studies. It supports 96-well formats, features intuitive software, and is optimized for both research and diagnostic applications.
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The Applied Biosystems 7500 Fast and 7500 Real-Time PCR Systems are versatile, high-performance platforms for the detection and quantification of nucleic acids in standard 96-well formats. Built on over a decade of real-time PCR expertise, these systems offer flexibility, speed, and reliability for a wide range of genetic analysis applications, including gene expression, SNP genotyping, and High Resolution Melting (HRM) analysis.
Key Features
Fast Results: 7500 Fast System delivers results in <30 minutes; 7500 System in <2 hours
Thermal System: Peltier-based 96-well block with 5.5°C (Fast) or 2.5°C (Standard) peak heating rate
Optics: CCD camera with halogen lamp, five-color detection with no need for new filters
Software (v2.0):
HRM Software support
Gene expression study tools
Comparative Ct (RQ), Allelic Discrimination, Standard Curve, and Plus/Minus applications
Plate Setup Wizard, real-time run monitoring, automatic email notifications
Data export to PowerPoint and JPEG
21 CFR Part 11 compliance-ready (customizable module)
Multiplexing: Up to 5 targets per well with multicomponent algorithm minimizing spectral crosstalk
Dye Compatibility: Supports FAM™, VIC®, JOE™, NED™, TAMRA™, Cy3®, ROX™, Texas Red®, SYBR® Green, Cy5®
Reaction Volumes:
7500 Fast: 5–30 µL
7500 Standard: 20–100 µL
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